Morphology of Dimorphic Fungi

Pathogenic fungi can exist as yeasts or as hyphae. A mass of hyphae is called mycelia.
Yeasts are unicellular organisms and mycelia are multicellular filamentous structures,
constituted by tubular cells with cell wall. The yeasts reproduce by budding. The mycelia
forms branch and the pattern of branching is an aid to the morphological identification. If
the mycelia do not have SEPTA, they are called coenocytic (nonseptate). The terms
“hypha” and “mycelium” are frequently used interchangeably. Some fungi occur in both
the yeast and mycelial forms. These are called dimorphic fungi.

Dimorphic Fungi

The dimorphic fungi have two forms
1. YEAST- (Parasitic or Pathogenic form). This sis the form usually seen in tissue,
in exudates, or cultured in an incubator at 370C .
2. MYCELIUM- (Saprophytic form). The form observed in nature or when cultured
at 260C. Conversion to the yeast form appears to be essential for pathogenicity. In
the dimorphic fungi, fungi are identified by several morphological or biochemical
characteristics, including the appearance of their fruiting bodies. The asexual
spores may be large (Macroconidia, Chlamydospores), or small (microconidia,
blastospores, arthroconidia).
There are four types of mycotic diseases:
1. Hypersensitivity- an allergic reaction to molds and spores
2. Mycotoxicoses- poisoning of man and animals by feeds and food products
contaminated by fungi which produce toxin from the grain substrate.
3. Mycetismus- the ingestion of toxin (mushroom poisoning)
4. Infection
We shall be concerned mainly with the last type; pathogenic fungi that cause infections.
Most common pathogenic fungi do not produce toxins but they do show physiologic
modifications during a parasitic infection ( e.g. increased metabolic rate, modified
metabolic pathways and modified cell wall structure). The mechanisms that cause these
modifications as well as their significance as a pathogenic mechanism are just being
described. Most pathogenic fungi are also thermo tolerant, and can resist the effects of
the active oxygen radicals released during the respiratory burst of phagocytes. Thus,
fungi are able to withstand many host defenses. Fungi are ubiquitous in nature and most
people are exposed to them. The establishment of mycotic infection usually depends on
the size of the inoculums and on the resistance of the host. The severity of the infection
seems to depend mostly on the immunologic status of the host. Thus, the demonstration
of fungi, for example, in blood drawn from an intravenous catheter can correspond to
colonization of the catheter, to transient fungemia (i.e. dissemination of fungi through
the blood stream), or to a true infection. The physician must decide which is the clinical
status of the patient based on clinical parameters, general status of the patient, laboratory
results, etc. the decision is not trivial, since treatment of systemic fungal infections
requires the aggressive use of drugs with considerable toxicity. Most mycotic agents are
soil saprophytes and mycotic diseases are generally not communicable from person-to24
person (occasional exceptions: candida and some dematophytes). Outbreaks of disease
may occur, but these are due to a common environmental exposure, not
communicability. Most of the fungi which cause systemic infections have a peculiar
characteristic ecologic niche in nature. This habitat is specific for several fungi which
will be discussed later. In this environment, the normally saprophytic organism
proliferate and develop. This habitat is also the source of fungal elements and/or spores,
where man and animals, incidental hosts, are exposed to the infectious particles. It is
important to be aware of these associations to diagnose mycotic diseases. The physicians
must be able to elicit a complete history from the patient including occupation, vocation
and travel history. This information is frequently required to raise, or confirm, your
differential diagnosis. The incidence of mycotic infections is currently increasing (in
man) dramatically, due to an immunosuppressive therapy, and the use of more invasive
diagnostic and surgical procedures (prosthetic implants). Fungal diseases are noncontagious
and non-reportable diseases in the national public health statistics.

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