I. Tissue Acquisition and Preparation
Under anesthesia, the
abdominal aorta, heart and one kidney are carefully removed from twelve rats
per strain and placed in a labeled specimen cup containing approximately 75 mls
of 10% Formaldehyde in phosphate buffer. The rostral end of the aorta is marked
with suture to insure that the caudal end will be sectioned. The tissues are
allowed to fix overnight at room temperature before they are handled. The
following day, the tissues are examined and the heart and kidney are bisected
before all three tissues are placed in labeled cassettes prior to automated
processing. The rostral-caudal length of the hearts are measured and noted.
They are then bisected at their mid-point to insure that sections will be taken
from an anatomically consistent level. The kidneys are also bisected at the
midsagittal plane to improve fixative penetration. The cassettes are returned
to the 10% Formaldehyde solution and are held under house vacuum for a minimum
of three days until they are ready to be loaded into the automatic tissue
processor.
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